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ASSERT is the only phase 3 study 
of an IBAT inhibitor in ALGS1

All images are actor portrayals.

ALGS: ASSERT Trial Program

Bylvay has been studied in the largest phase 3 clinical trial program in cholestatic pruritus of ALGS and PFIC to date1-3

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32

sites

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13

countries
participated

A double-blind, randomized, placebo-controlled study with a 24-week treatment period followed by an ongoing, 
open-label, 72-week extension

aseert

ASSERT 1 • 24 WEEKS1,3

Double-blind, randomized, placebo-controlled

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Primary Endpoint 1,3,6

Change in pruritus from baseline to month 6 (weeks 21-24)

Secondary Endpoints 1,3,6

  • Change in sBA levels through week 24
  • Change in sleep parameters through
    week 24
  • Change in global symptom relief* at
    weeks 4, 12, and 24

ASSERT 2 • 72 WEEKS1,4,5

Ongoing open-label extension

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Primary Endpoint4

Change in pruritus through week 72

Secondary Endpoints4

  • Change in sBA levels through week 72
  • Change in sleep parameters through week 72
  • Change in global symptom relief* at weeks 4, 12, 24, 48, and 72

Concomitant UDCA and other antipruritic medications were permitted.6

*Change from baseline in global symptom relief as measured by the patient, caregiver, and clinician on the GIS (PGIS, CaGIS, CGIS) and the GIC (PGIC, CaGIC, CGIC) items.7

ALGS=Alagille syndrome; CaGIC=Caregiver Global Impression of Change; CaGIS=Caregiver Global Impression of Symptoms; CGIC=Clinical Global Impression of Change; CGIS=Clinical Global Impression of Symptoms; GIC=Global Impression of Change; GIS=Global Impression of Symptoms; IBAT=ileal bile acid transporter; PFIC=progressive familial intrahepatic cholestasis; PGIC=Patient Global Impression of Change; PGIS=Patient Global Impression of Symptoms; sBA=serum bile acid; UDCA=ursodeoxycholic acid.

References:
  • Bylvay Prescribing Information. Boston, MA: Albireo Pharma, Inc.; 2023.
  • Kamath BM, Stein P, Houwen RHJ, Verkade HJ. Potential of ileal bile acid transporter inhibition as a therapeutic target in Alagille syndrome and progressive familial intrahepatic    
    cholestasis. Liver Int. 2020;40(8):1812-1822.
  • ClinicalTrials.gov. A phase 3 double-blind, randomized, placebo-controlled study of the safety and efficacy of odevixibat (A4250) in patients with Alagille syndrome (ASSERT).    
    NCT04674761. Updated April 10, 2023. Accessed April 24, 2023.
  • ClinicalTrials.gov. An open-label study to evaluate the long-term safety and efficacy of odevixibat (A4250) in patients with Alagille syndrome (ASSERT-EXT). NCT05035030. Updated   
    October 18, 2022. Accessed April 24, 2023.
  • Data on file A4250-015. November 11, 2022. Boston, MA: Albireo Pharma, Inc.
  • Data on file A4250-012. November 10, 2022. Boston, MA: Albireo Pharma, Inc.
  • Gwaltney C, Ivanescu C, Karlsson L, Warholic N, Kjems L, Horn P. Validation of the PRUCISION instruments in pediatric patients with progressive familial intrahepatic cholestasis. Adv   
    Ther. 2022;39:5105-5125.
  • Bylvay Prescribing Information. Boston, MA: Albireo Pharma, Inc.; 2023.
  • Kamath BM, Stein P, Houwen RHJ, Verkade HJ. 
    Potential of ileal bile acid transporter inhibition as a 
    therapeutic target in Alagille syndrome and 
    progressive familial intrahepatic cholestasis. Liver Int. 
    2020;40(8):1812-1822.
  • ClinicalTrials.gov. A phase 3 double-blind, randomized, placebo-controlled study of the safety and efficacy of odevixibat (A4250) in patients with Alagille syndrome (ASSERT). NCT04674761. Updated April 10, 2023. Accessed April 24, 2023.
  • ClinicalTrials.gov. An open-label study to evaluate the long-term safety and efficacy of odevixibat (A4250) in patients with Alagille syndrome (ASSERT-EXT). NCT05035030. Updated October 18, 2022. Accessed April 24, 2023.
  • Data on file A4250-015. November 11, 2022. Boston, MA: Albireo Pharma, Inc.
  • Data on file A4250-012. November 10, 2022. Boston, MA: Albireo Pharma, Inc.
  • Gwaltney C, Ivanescu C, Karlsson L, Warholic N, Kjems 
    L, Horn P. Validation of the PRUCISION instruments in 
    pediatric patients with progressive familial intrahepatic 
    cholestasis. Adv  Ther. 2022;39:5105-5125.